rabbit anti synaptopodin antibody  (Proteintech)

Journal: NPJ Parkinson's Disease

Article Title: Advancing age and the rs6265 BDNF SNP are permissive to graft-induced dyskinesias in parkinsonian rats

doi: 10.1038/s41531-024-00771-6

Figure Lengend Snippet: Antigen targets and corresponding antibodies

Article Snippet: TH-Synaptopodin , Synaptopodin , rabbit anti-synaptopodin , 1:4000 , Synaptic Systems , 163002 , A21207.

Techniques:

Journal: bioRxiv

Article Title: Cell-surface receptor-mediated regulation of synaptic organelle distribution controls dendritic spine maturation

doi: 10.1101/2024.05.08.592949

Figure Lengend Snippet: PLCXD2 hinders SA formation (see also Supplementary figure S5). (A) DIV16 mouse cultured hippocampal neuron co-expressing PLCXD2-FLAG and GFP immunostained for FLAG (red), GFP (gray) and F-actin (cyan). Scale bar 10 µm, inset 5 µm. (B) Representative images of dendritic spines from cultured hippocampal neurons lacking or containing PLCXD2-FLAG, immunostained for F-actin (cyan). Scale bar 1 µm. (C) Quantification of F-actin fluorescence intensity in spines. Each pair of dot plots represents the average F-actin fluorescence intensity in PLCXD2- and PLCXD2+ spines from the same neuron ( n = 3 cultures, 13 neurons). *** P <0.001, Wilcoxon matched-pairs signed rank test. (D) DIV16 mouse cultured hippocampal neuron co-expressing PLCXD2-FLAG and GFP were immunostained for FLAG (red), GFP (gray) and SYNPO (cyan). Scale bar 10 µm, inset 5 µm. (E) Percentage of PLCXD2+ spines containing SYNPO and vice versa. (F) Quantification of SYNPO fluorescence intensity. Each pair of dot plots represents the average SYNPO fluorescence intensity in PLCXD2- and PLCXD2+ spines from the same neuron ( n = 3 cultures, 14 neurons). **** P <0.0001, two-tailed paired t -test.

Article Snippet: Primary antibodies were the following: chicken anti-GFP (1:1000, Aves Labs), rat anti-HA (1:250, Roche), mouse anti-β-galactosidase (1:1000, Roche), rabbit anti- GPR158 (1:200, Sigma-Aldrich), rabbit anti-synaptopodin SE19 (1:500, Sigma- Aldrich), camelid PSD95-FluoTag-X2 (1:500, Synaptic Systems), guinea-pig anti- VGluT1 (1:000, Synaptic Systems), and guinea-pig anti-VGluT2 (1:1000, Synaptic Systems).

Techniques: Cell Culture, Expressing, Fluorescence, Two Tailed Test

Journal: bioRxiv

Article Title: Cell-surface receptor-mediated regulation of synaptic organelle distribution controls dendritic spine maturation

doi: 10.1101/2024.05.08.592949

Figure Lengend Snippet: A GPR158-PLCXD2 complex regulates SA content and maturation of dendritic spines (see also Supplementary figure S7). (A) Experimental strategy to achieve simultaneous sparse gene cKO and cell labeling. Leakage of the TRE promoter drives weak expression of Cre and, subsequently, tTA in a very small population of neurons that carry both vectors. Next, tTA enhances Cre expression by binding to TRE. This positive feedback of successive tTA-TRE cycles facilitates expression of Cre and GFP in the scarce population of transduced neurons. (B-D) Representative images of dendritic segments from WT (B) , Gpr158 cKO (C) and Gpr158 : Plcxd2 dcKO (D) neurons in L4 of the barrel cortex, immunostained for GFP and SYNPO at P14. Scale bar 5 µm. ( E) Quantification of dendritic spine morphology in WT (N = 3 mice, n = 9 neurons), Gpr158 cKO ( N = 3 mice, n = 9 neurons) and Gpr158 : Plcxd2 dcKO neurons ( N = 3 mice, n = 9 neurons). ** P <0.001, *** P <0.001, nested 1-way ANOVA, Tukey’s multiple comparisons. (F) Quantification of SYNPO-containing spines in WT (N = 3 mice, n = 9 neurons), Gpr158 cKO ( N = 3 mice, n = 9 neurons) and Gpr158 : Plcxd2 dcKO neurons ( N = 3 mice, n = 9 neurons). **** P <0.0001, nested 1-way ANOVA, Tukey’s multiple comparisons. (G) Quantification of SYNPO-containing mushroom-type spines in WT ( N = 3 mice, n = 9 neurons), Gpr158 cKO ( N = 3 mice, n = 9 neurons) and Gpr158 : Plcxd2 dcKO neurons ( N = 3 mice, n = 9 neurons). * P <0.05, nested 1-way ANOVA, Tukey’s multiple comparisons. (H) Quantification of dendritic spine density in WT ( N = 3 mice, n = 9 neurons), Gpr158 cKO ( N = 3 mice, n = 9 neurons) and Gpr158 : Plcxd2 dcKO neurons ( N = 3 mice, n = 9 neurons).

Article Snippet: Primary antibodies were the following: chicken anti-GFP (1:1000, Aves Labs), rat anti-HA (1:250, Roche), mouse anti-β-galactosidase (1:1000, Roche), rabbit anti- GPR158 (1:200, Sigma-Aldrich), rabbit anti-synaptopodin SE19 (1:500, Sigma- Aldrich), camelid PSD95-FluoTag-X2 (1:500, Synaptic Systems), guinea-pig anti- VGluT1 (1:000, Synaptic Systems), and guinea-pig anti-VGluT2 (1:1000, Synaptic Systems).

Techniques: Labeling, Expressing, Binding Assay